Journal: International Journal of Molecular Sciences

Article Title: Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes

doi: 10.3390/ijms20133324

Figure Lengend Snippet: Scratching significantly upregulates the expression of IL13RA2 in NHEK cells. A confluent keratinocyte culture was scratched with 14 lines, and the expression of IL13RA2 , IL4R , and IL13RA1 was analyzed by qRT-PCR and normalized to that of β-actin. Scratching significantly increased IL13RA2 expression in NHEK cells ( A ). IL4R ( B ) and IL13RA1 ( C ) expression was not altered. The cells were incubated for 6 h after scratching. Data is shown as the mean ± SEM ( n = 3). *** p < 0.001.

Article Snippet: Plasmids pCMV6-Entry (Mock) and IL13RA2 (Myc-DDK-tagged), which contains a cytomegalovirus promoter, and the IL13RA2 (NM_000640) human cDNA open reading frame clone were obtained from Origene Technologies (Rockville, MD, USA).

Techniques: Expressing, Quantitative RT-PCR, Incubation

Journal: International Journal of Molecular Sciences

Article Title: Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes

doi: 10.3390/ijms20133324

Figure Lengend Snippet: Time-course study for IL13RA2 ( A ), IL4R ( B ), and IL13RA1 ( C ) expression. The gene expression of IL13RA2 , IL4R , and IL13RA1 was measured with or without scratching at 0, 3, 6, 9, 12, and 24 h ( n = 3). Data is shown as the mean ± SEM. ns: not significant. *** p < 0.001.

Article Snippet: Plasmids pCMV6-Entry (Mock) and IL13RA2 (Myc-DDK-tagged), which contains a cytomegalovirus promoter, and the IL13RA2 (NM_000640) human cDNA open reading frame clone were obtained from Origene Technologies (Rockville, MD, USA).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes

doi: 10.3390/ijms20133324

Figure Lengend Snippet: Scratching upregulated IL13RA2 expression in a scratch line number-dependent manner. The keratinocyte sheet was scratched with 7, 14, and 18 scratch lines, and the gene expression of IL13RA2 ( A ), IL4R ( B ), and IL13RA1 ( C ) was measured ( n = 3) 6 h after scratching. Data is shown as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Plasmids pCMV6-Entry (Mock) and IL13RA2 (Myc-DDK-tagged), which contains a cytomegalovirus promoter, and the IL13RA2 (NM_000640) human cDNA open reading frame clone were obtained from Origene Technologies (Rockville, MD, USA).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes

doi: 10.3390/ijms20133324

Figure Lengend Snippet: The effect of IL-13 on scratch-induced IL13RA2 ( A ), IL4R ( B ), and IL13RA1 ( C ) expression. Confluent keratinocyte sheets were non-scratched or scratched with 18 lines in the presence or absence of graded IL-13 concentrations (1, 5, 10 ng/mL). Cells were treated with IL-13 for 14 h before scratching and then incubated for another 6 h. Data is shown as the mean ± SEM. * p < 0.05, *** p < 0.001.

Article Snippet: Plasmids pCMV6-Entry (Mock) and IL13RA2 (Myc-DDK-tagged), which contains a cytomegalovirus promoter, and the IL13RA2 (NM_000640) human cDNA open reading frame clone were obtained from Origene Technologies (Rockville, MD, USA).

Techniques: Expressing, Incubation

Journal: International Journal of Molecular Sciences

Article Title: Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes

doi: 10.3390/ijms20133324

Figure Lengend Snippet: The effect of MAPK inhibitors on IL13RA2 ( A ), IL4R ( B ), and IL13RA1 ( C ) expression. Non-scratched and scratched confluent keratinocytes were treated with or without U0126 (MEK 1/2-ERK1/2 inhibitor), SP600125 (JNK inhibitor), and SB203580 (p38MAPK inhibitor). Data is shown as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Plasmids pCMV6-Entry (Mock) and IL13RA2 (Myc-DDK-tagged), which contains a cytomegalovirus promoter, and the IL13RA2 (NM_000640) human cDNA open reading frame clone were obtained from Origene Technologies (Rockville, MD, USA).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes

doi: 10.3390/ijms20133324

Figure Lengend Snippet: IL13RA2 expression was upregulated in the IL-13Rα2-Tg-HaCaT cells more than in control Moc-HaCaT cells ( A ). Upregulated IL-13Rα2 protein expression was observed in the IL-13Rα2-Tg-HaCaT cells, compared to that in Moc-HaCaT cells ( B ). IL-13-induced IVL downregulation was partially restored in IL-13Rα2-Tg-HaCaT cells ( C ). ** p < 0.01, *** p < 0.001.

Article Snippet: Plasmids pCMV6-Entry (Mock) and IL13RA2 (Myc-DDK-tagged), which contains a cytomegalovirus promoter, and the IL13RA2 (NM_000640) human cDNA open reading frame clone were obtained from Origene Technologies (Rockville, MD, USA).

Techniques: Expressing

Journal: Pharmaceuticals

Article Title: Drug Conjugates for Targeting Eph Receptors in Glioblastoma

doi: 10.3390/ph13040077

Figure Lengend Snippet: QUAD 3.0-WP936 binding to EphA3, EphA2, EphB2, and IL-13RA2 receptors. ELISA assay showing the binding of unconjugated QUAD 3.0 and QUAD 3.0-WP936 conjugate to ( a ) EphA3; ( b ) EphA2; ( c ) EphB2; and ( d ) IL-13RA2 receptor proteins.

Article Snippet: First, 96-well ELISA plates were coated by incubation at 4 °C overnight with 100 μL of 1mg/mL of human recombinant EphA3, EphA2, EphB2, or IL-13RA2 (Sino Biological, Wayne, PA, USA).

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay

Journal: Pharmaceuticals

Article Title: Drug Conjugates for Targeting Eph Receptors in Glioblastoma

doi: 10.3390/ph13040077

Figure Lengend Snippet: Kd and Bmax values of QUAD 3.0 and the QUAD 3.0 Dox conjugates binding to the EphA3, EphA2, EphB2, and IL-13RA2 receptors.

Article Snippet: First, 96-well ELISA plates were coated by incubation at 4 °C overnight with 100 μL of 1mg/mL of human recombinant EphA3, EphA2, EphB2, or IL-13RA2 (Sino Biological, Wayne, PA, USA).

Techniques: Binding Assay

Journal: Pharmaceuticals

Article Title: Drug Conjugates for Targeting Eph Receptors in Glioblastoma

doi: 10.3390/ph13040077

Figure Lengend Snippet: QUAD 3.0-WP1737 binding to EphA3, EphA2, EphB2, and IL-13RA2 receptors. ELISA assay showing the binding of unconjugated QUAD 3.0 and QUAD 3.0-WP1737 conjugate to ( a ) EphA3; ( b ) EphA2; ( c ) EphB2; and ( d ) IL-13RA2 receptor proteins.

Article Snippet: First, 96-well ELISA plates were coated by incubation at 4 °C overnight with 100 μL of 1mg/mL of human recombinant EphA3, EphA2, EphB2, or IL-13RA2 (Sino Biological, Wayne, PA, USA).

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay

Journal: Pharmaceuticals

Article Title: Drug Conjugates for Targeting Eph Receptors in Glioblastoma

doi: 10.3390/ph13040077

Figure Lengend Snippet: QUAD 3.0 Dox 1244 binding to EphA3, EphA2, EphB2, and IL-13RA2 receptors. ELISA results showing the binding of unconjugated QUAD 3.0 and QUAD 3.0-WP1244 conjugate to ( a ) EphA3; ( b ) EphA2; ( c ) EphB2; and ( d ) IL-13RA2 receptor proteins.

Article Snippet: First, 96-well ELISA plates were coated by incubation at 4 °C overnight with 100 μL of 1mg/mL of human recombinant EphA3, EphA2, EphB2, or IL-13RA2 (Sino Biological, Wayne, PA, USA).

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay

Journal: Cell reports

Article Title: TGF-β1-mediated intercellular signaling fuels cooperative cellular invasion

doi: 10.1016/j.celrep.2025.115315

Figure Lengend Snippet:

Article Snippet: CD213a2 (IL13Ra2) (APC) , Miltenyi Biotec , Cat#130-128-220; RRID: AB_2928320.

Techniques: Recombinant, Reverse Transcription, Bicinchoninic Acid Protein Assay, Staining, RNA Sequencing

Journal: Neuro-oncology Advances

Article Title: Multireceptor targeting of glioblastoma

doi: 10.1093/noajnl/vdaa107

Figure Lengend Snippet: IL-13RA2, EphA2, and EphA3 receptors cover conjointly nearly 100% of the heterogeneous glioblastoma (GBM) microenvironment. Immunofluorescent staining of IL-13RA2 (purple), EphA2 (red), EphA3 (green) and nucleus (4′,6-diamidino-2-phenylindole [DAPI], blue) in sections of 2 GBM specimens.

Article Snippet: Human recombinant IL-13RA2, EphA2, EphA3, and EphB2 proteins (Sino Biologicals) were used to coat the ELISA plates.

Techniques: Staining

Journal: Neuro-oncology Advances

Article Title: Multireceptor targeting of glioblastoma

doi: 10.1093/noajnl/vdaa107

Figure Lengend Snippet: Distribution of the 4 Pharmaceutically Targetable Receptors Within the GBM Microenvironment

Article Snippet: Human recombinant IL-13RA2, EphA2, EphA3, and EphB2 proteins (Sino Biologicals) were used to coat the ELISA plates.

Techniques:

Journal: Neuro-oncology Advances

Article Title: Multireceptor targeting of glioblastoma

doi: 10.1093/noajnl/vdaa107

Figure Lengend Snippet: KD Values of QUAD Variants Binding to the IL-13RA2, EphA2, EphA3, and EphB2 Receptors

Article Snippet: Human recombinant IL-13RA2, EphA2, EphA3, and EphB2 proteins (Sino Biologicals) were used to coat the ELISA plates.

Techniques: Binding Assay

Journal: Neuro-oncology Advances

Article Title: Multireceptor targeting of glioblastoma

doi: 10.1093/noajnl/vdaa107

Figure Lengend Snippet: QUAD variants retain the functional properties of their individual ligands. (A) QUADs binding to the targeted receptors. ELISA of QUAD 1.0 (i), QUAD 2.0 (ii), and QUAD 3.0 (iii) binding to IL-13RA2, EphB2, EphA2, and EphA3 receptors. (B) and (C) Downregulation of the EphA3 and EphA2 receptors by the QUADs. Western blot of the EphA3 and EphA2 receptors upon exposure to 1 µg/mL of individual QUADs in U-251 GBM cells.

Article Snippet: Human recombinant IL-13RA2, EphA2, EphA3, and EphB2 proteins (Sino Biologicals) were used to coat the ELISA plates.

Techniques: Functional Assay, Binding Assay, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Neuro-oncology Advances

Article Title: Multireceptor targeting of glioblastoma

doi: 10.1093/noajnl/vdaa107

Figure Lengend Snippet: QUAD 3.0-PE38QQR conjugate retains the binding ability to the 4 targeted receptors. Comparative binding of QUAD 3.0 and QUAD 3.0-PE38QQR conjugate to (A) EphA3, (B) EphA2, (C) IL-13RA2, and (D) EphB2 receptors.

Article Snippet: Human recombinant IL-13RA2, EphA2, EphA3, and EphB2 proteins (Sino Biologicals) were used to coat the ELISA plates.

Techniques: Binding Assay